Composite

Part:BBa_K4390054

Designed by: Maarten van den Ancker   Group: iGEM22_Edinburgh-UHAS_Ghana   (2022-09-03)


Lead biosensor (in plasmid)

Functions the same as the linear lead biosensor (BBa_K4390050), except has a T7 terminator so can be used in circular DNA.


Please refer to https://parts.igem.org/Part:BBa_K4390051 for more details on how this biosensor functions.

Sequence and Features


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal SpeI site found at 258
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal SpeI site found at 258
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal SpeI site found at 258
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal SpeI site found at 258
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 134


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Categories
Parameters
None